Welcome to A4liveCell Blog #6!
SPAchip® as an image-based real-time readout of metabolic activity in genetic screening
Understanding how the genetic information give rise to the phenotype, and how genomic perturbation affects a given phenotype are some of the main objectives of genetic screening. Recently, microscopy or FACS-based screening have emerged as new option to unravel different aspects of cell biology. This blog dives into how genetic screening approaches in combination with SPAchip® technology can be use to understand the metabolism for fundamental biology or identification of molecular targets of drug candidates.
Mapping Genotypes to phenotypes by image-based genetic screening
Image-based profiling captures intricate spatial and morphological changes in cells, providing a rich dataset that complements next-generation sequencing (NGS). This approach allows for the simultaneous analysis of multiple phenotypic markers through multiplexed imaging, offering a comprehensive view of genetic modifications’ effects on cellular structures and functions.
Different kinds of image-based genetic screenings include:
- Arrayed Screens: Each well contains a specific mutant whose phenotype can be assessed.
- Pooled Enrichment Screens: Individual cell genomes are disrupted in a single sample, followed by phenotypic enrichment and abundance estimation via NGS.
SPAchip® serves as an image-based, real-time readout of metabolic activity, making it an invaluable tool for genetic screening. It can dynamically measure key regulatory elements like pH, ROS, and Ca2+ using its broad range of fluorescent emissions. This versatility supports both arrayed and pooled enrichment screens, potentially substituting traditional FACS with high-speed fluorescence imaging methods.
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