Products

CytoCHECK SPAchip® assay kits are novel fluorescence assays developed by A4Cell that brings together the fields of nanotechnology and cell biology.

CytoCHECK SPAchip™ are composed of fluorescently labeled silicon microparticles -SPAchips®- that can be internalized in the cytosol of cultured cells and monitor changes for long periods of time.
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CytoCHECK SPAchip® kits are optimized for its use with microscopes and HCS/HCA analyzers with confocal capability and 40X or over magnification objectives, yet epi-fluorescence microscopes and imaging system with fixed wavelength filters can be also used. SPAchips® are multiplexed with an array of arranged fluorescent spots that respond to different stimuli and whose fluorescence can be easily visualized and measured. Depending on the kit, wavelengths and probes will vary.

SPAchips are used as common fluorophores and chemical probes for cell biology. After resuspending them in the assay buffer, simply add 1 µl per well to the cells in a 96-well plate and incubate overnight to let the chips being incorporated in the cytosol. SPAchips® will remain in the cytosol for longer than one month to monitor the evolution of the culture.